PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar.
Identifieur interne : 003150 ( Main/Exploration ); précédent : 003149; suivant : 003151PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar.
Auteurs : Jérémy Couturier [France] ; Elisabeth De Fa ; Michael Fitz ; Daniel Wipf ; Damien Blaudez ; Michel ChalotSource :
- Journal of experimental botany [ 1460-2431 ] ; 2010.
Descripteurs français
- KwdFr :
- Différenciation cellulaire (génétique), Différenciation cellulaire (physiologie), Phylogenèse (MeSH), Populus (cytologie), Populus (génétique), Populus (métabolisme), RT-PCR (MeSH), Régulation de l'expression des gènes végétaux (génétique), Régulation de l'expression des gènes végétaux (physiologie), Spécificité du substrat (MeSH), Systèmes de transport d'acides aminés (classification), Systèmes de transport d'acides aminés (génétique), Systèmes de transport d'acides aminés (métabolisme), Xylème (cytologie), Xylème (génétique), Xylème (métabolisme).
- MESH :
- classification : Systèmes de transport d'acides aminés.
- cytologie : Populus, Xylème.
- génétique : Différenciation cellulaire, Populus, Régulation de l'expression des gènes végétaux, Systèmes de transport d'acides aminés, Xylème.
- métabolisme : Populus, Systèmes de transport d'acides aminés, Xylème.
- physiologie : Différenciation cellulaire, Régulation de l'expression des gènes végétaux.
- Phylogenèse, RT-PCR, Spécificité du substrat.
English descriptors
- KwdEn :
- Amino Acid Transport Systems (classification), Amino Acid Transport Systems (genetics), Amino Acid Transport Systems (metabolism), Cell Differentiation (genetics), Cell Differentiation (physiology), Gene Expression Regulation, Plant (genetics), Gene Expression Regulation, Plant (physiology), Phylogeny (MeSH), Populus (cytology), Populus (genetics), Populus (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Substrate Specificity (MeSH), Xylem (cytology), Xylem (genetics), Xylem (metabolism).
- MESH :
- chemical , classification : Amino Acid Transport Systems.
- chemical , genetics : Amino Acid Transport Systems.
- chemical , metabolism : Amino Acid Transport Systems.
- cytology : Populus, Xylem.
- genetics : Cell Differentiation, Gene Expression Regulation, Plant, Populus, Xylem.
- metabolism : Populus, Xylem.
- physiology : Cell Differentiation, Gene Expression Regulation, Plant.
- Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Substrate Specificity.
Abstract
Amino acids are the currency of nitrogen exchange between source and sink tissues in plants and constitute a major source of the components used for cellular growth and differentiation. The characterization of a new amino acid transporter belonging to the amino acid permease (AAP) family, AAP11, expressed in the perennial species Populus trichocarpa is reported here. PtAAP11 expression analysis was performed by semi-quantitative RT-PCR and GUS activity after poplar transformation. PtAAP11 function was studied in detail by heterologous expression in yeast. The poplar genome contains 14 putative AAPs which is quite similar to other species analysed except Arabidopsis. PtAAP11 was mostly expressed in differentiating xylem cells in different organs. Functional characterization demonstrated that PtAAP11 was a high affinity amino acid transporter, more particularly for proline. Compared with other plant amino acid transporters, PtAAP11 represents a novel high-affinity system for proline. Thus, the functional characterization and expression studies suggest that PtAAP11 may play a major role in xylogenesis by providing proline required for xylem cell wall proteins. The present study provides important information highlighting the role of a specific amino acid transporter in xylogenesis in poplar.
DOI: 10.1093/jxb/erq036
PubMed: 20190041
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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The characterization of a new amino acid transporter belonging to the amino acid permease (AAP) family, AAP11, expressed in the perennial species Populus trichocarpa is reported here. PtAAP11 expression analysis was performed by semi-quantitative RT-PCR and GUS activity after poplar transformation. PtAAP11 function was studied in detail by heterologous expression in yeast. The poplar genome contains 14 putative AAPs which is quite similar to other species analysed except Arabidopsis. PtAAP11 was mostly expressed in differentiating xylem cells in different organs. Functional characterization demonstrated that PtAAP11 was a high affinity amino acid transporter, more particularly for proline. Compared with other plant amino acid transporters, PtAAP11 represents a novel high-affinity system for proline. Thus, the functional characterization and expression studies suggest that PtAAP11 may play a major role in xylogenesis by providing proline required for xylem cell wall proteins. The present study provides important information highlighting the role of a specific amino acid transporter in xylogenesis in poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20190041</PMID><DateCompleted><Year>2010</Year><Month>07</Month><Day>01</Day></DateCompleted><DateRevised><Year>2010</Year><Month>04</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>61</Volume><Issue>6</Issue><PubDate><Year>2010</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar.</ArticleTitle><Pagination><MedlinePgn>1671-82</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erq036</ELocationID><Abstract><AbstractText>Amino acids are the currency of nitrogen exchange between source and sink tissues in plants and constitute a major source of the components used for cellular growth and differentiation. The characterization of a new amino acid transporter belonging to the amino acid permease (AAP) family, AAP11, expressed in the perennial species Populus trichocarpa is reported here. PtAAP11 expression analysis was performed by semi-quantitative RT-PCR and GUS activity after poplar transformation. PtAAP11 function was studied in detail by heterologous expression in yeast. The poplar genome contains 14 putative AAPs which is quite similar to other species analysed except Arabidopsis. PtAAP11 was mostly expressed in differentiating xylem cells in different organs. Functional characterization demonstrated that PtAAP11 was a high affinity amino acid transporter, more particularly for proline. Compared with other plant amino acid transporters, PtAAP11 represents a novel high-affinity system for proline. Thus, the functional characterization and expression studies suggest that PtAAP11 may play a major role in xylogenesis by providing proline required for xylem cell wall proteins. The present study provides important information highlighting the role of a specific amino acid transporter in xylogenesis in poplar.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Couturier</LastName><ForeName>Jérémy</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>UMR INRA/UHP 1136 Interactions Arbres-Microorganismes, IFR 110 Ecosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation, Nancy University, Faculté des Sciences et Techniques, BP 70239, F-54506 Vandoeuvre-les-Nancy Cedex, France. Jeremy.Couturier@scbiol.uhp-nancy.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Faÿ</LastName><ForeName>Elisabeth</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Fitz</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Wipf</LastName><ForeName>Daniel</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Blaudez</LastName><ForeName>Damien</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Chalot</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>02</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026905">Amino Acid Transport Systems</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D026905" MajorTopicYN="N">Amino Acid Transport Systems</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20190041</ArticleId><ArticleId IdType="pii">erq036</ArticleId><ArticleId IdType="doi">10.1093/jxb/erq036</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Vandœuvre-lès-Nancy</li></settlement></list><tree><noCountry><name sortKey="Blaudez, Damien" sort="Blaudez, Damien" uniqKey="Blaudez D" first="Damien" last="Blaudez">Damien Blaudez</name><name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name><name sortKey="De Fa, Elisabeth" sort="De Fa, Elisabeth" uniqKey="De Fa E" first="Elisabeth" last="De Fa">Elisabeth De Fa</name><name sortKey="Fitz, Michael" sort="Fitz, Michael" uniqKey="Fitz M" first="Michael" last="Fitz">Michael Fitz</name><name sortKey="Wipf, Daniel" sort="Wipf, Daniel" uniqKey="Wipf D" first="Daniel" last="Wipf">Daniel Wipf</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Couturier, Jeremy" sort="Couturier, Jeremy" uniqKey="Couturier J" first="Jérémy" last="Couturier">Jérémy Couturier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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